Coding

Part:BBa_K4769212:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


nprB coding sequence from B. subtilis 3610


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 320
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 320
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 320
    Illegal BamHI site found at 270
    Illegal XhoI site found at 472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 320
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 320
    Illegal NgoMIV site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: ttgcgcaacttgaccaaga (+ desired overhangs) RV: tcatagaatgccgacagcct (+ desired overhangs)


References